PoplarV1, Main, Exploration, bibRecord, 002633

In situ hybridization.

Identifieur interne : 002633 ( Main/Exploration ); précédent : 002632; suivant : 002634

In situ hybridization.

Auteurs : Chung-Jui Tsai [États-Unis] ; Scott A. Harding

Source :

Methods in cell biology [ 0091-679X ] ; 2013.

RBID : pubmed:23317910

Descripteurs français

KwdFr :
- ARN de transfert (composition chimique), ARN des plantes (génétique), ARN des plantes (métabolisme), ARN fongique (composition chimique), ARN messager (génétique), ARN messager (métabolisme), Alcool tert-butylique (composition chimique), Coloration et marquage (MeSH), Eucalyptus (génétique), Eucalyptus (métabolisme), Fixateurs (composition chimique), Fixation tissulaire (MeSH), Formaldéhyde (composition chimique), Hybridation in situ (méthodes), Inclusion en paraffine (MeSH), Microtomie (MeSH), Polymères (composition chimique), Populus (génétique), Populus (métabolisme), Protéines végétales (génétique), Protéines végétales (métabolisme), Solutions (MeSH), Sondes d'ARN (composition chimique), Substances tampon (MeSH).
MESH :
- composition chimique : ARN de transfert, ARN fongique, Alcool tert-butylique, Fixateurs, Formaldéhyde, Polymères, Sondes d'ARN.
- génétique : ARN des plantes, ARN messager, Eucalyptus, Populus, Protéines végétales.
- métabolisme : ARN des plantes, ARN messager, Eucalyptus, Populus, Protéines végétales.
- méthodes : Hybridation in situ.
- Coloration et marquage, Fixation tissulaire, Inclusion en paraffine, Microtomie, Solutions, Substances tampon.

English descriptors

KwdEn :
- Buffers (MeSH), Eucalyptus (genetics), Eucalyptus (metabolism), Fixatives (chemistry), Formaldehyde (chemistry), In Situ Hybridization (methods), Microtomy (MeSH), Paraffin Embedding (MeSH), Plant Proteins (genetics), Plant Proteins (metabolism), Polymers (chemistry), Populus (genetics), Populus (metabolism), RNA Probes (chemistry), RNA, Fungal (chemistry), RNA, Messenger (genetics), RNA, Messenger (metabolism), RNA, Plant (genetics), RNA, Plant (metabolism), RNA, Transfer (chemistry), Solutions (MeSH), Staining and Labeling (MeSH), Tissue Fixation (MeSH), tert-Butyl Alcohol (chemistry).
MESH :
- chemical , chemistry : Fixatives, Formaldehyde, Polymers, RNA Probes, RNA, Fungal, RNA, Transfer, tert-Butyl Alcohol.
- chemical , genetics : Plant Proteins, RNA, Messenger, RNA, Plant.
- chemical , metabolism : Plant Proteins, RNA, Messenger, RNA, Plant.
- chemical : Buffers, Solutions.
- genetics : Eucalyptus, Populus.
- metabolism : Eucalyptus, Populus.
- methods : In Situ Hybridization.
- Microtomy, Paraffin Embedding, Staining and Labeling, Tissue Fixation.

Abstract

A non-radioactive in situ hybridization protocol is presented for localization of mRNA transcripts in thin tissue sections. In situ hybridization provides spatial resolution of transcript distribution at the cellular level that is not attained by Northern hybridization or PCR-based methods using organ- or tissue-derived RNA. With appropriately designed gene-specific probes, in situ hybridization can discriminate closely related gene family members. Although the procedure for tissue section preparation is tedious and lengthy, once prepared, the probe labeling, hybridization and signal detection steps can be easily scaled to handle multiple genes for comparative analysis.

DOI: 10.1016/B978-0-12-407239-8.00016-1
PubMed: 23317910

Affiliations:

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Le document en format XML

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<author><name sortKey="Tsai, Chung Jui" sort="Tsai, Chung Jui" uniqKey="Tsai C" first="Chung-Jui" last="Tsai">Chung-Jui Tsai</name>
<affiliation wicri:level="2"><nlm:affiliation>Warnell School of Forestry and Natural Resources, and Department of Genetics, University of Georgia, GA, USA.</nlm:affiliation>
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<author><name sortKey="Harding, Scott A" sort="Harding, Scott A" uniqKey="Harding S" first="Scott A" last="Harding">Scott A. Harding</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Buffers (MeSH)</term>
<term>Eucalyptus (genetics)</term>
<term>Eucalyptus (metabolism)</term>
<term>Fixatives (chemistry)</term>
<term>Formaldehyde (chemistry)</term>
<term>In Situ Hybridization (methods)</term>
<term>Microtomy (MeSH)</term>
<term>Paraffin Embedding (MeSH)</term>
<term>Plant Proteins (genetics)</term>
<term>Plant Proteins (metabolism)</term>
<term>Polymers (chemistry)</term>
<term>Populus (genetics)</term>
<term>Populus (metabolism)</term>
<term>RNA Probes (chemistry)</term>
<term>RNA, Fungal (chemistry)</term>
<term>RNA, Messenger (genetics)</term>
<term>RNA, Messenger (metabolism)</term>
<term>RNA, Plant (genetics)</term>
<term>RNA, Plant (metabolism)</term>
<term>RNA, Transfer (chemistry)</term>
<term>Solutions (MeSH)</term>
<term>Staining and Labeling (MeSH)</term>
<term>Tissue Fixation (MeSH)</term>
<term>tert-Butyl Alcohol (chemistry)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ARN de transfert (composition chimique)</term>
<term>ARN des plantes (génétique)</term>
<term>ARN des plantes (métabolisme)</term>
<term>ARN fongique (composition chimique)</term>
<term>ARN messager (génétique)</term>
<term>ARN messager (métabolisme)</term>
<term>Alcool tert-butylique (composition chimique)</term>
<term>Coloration et marquage (MeSH)</term>
<term>Eucalyptus (génétique)</term>
<term>Eucalyptus (métabolisme)</term>
<term>Fixateurs (composition chimique)</term>
<term>Fixation tissulaire (MeSH)</term>
<term>Formaldéhyde (composition chimique)</term>
<term>Hybridation in situ (méthodes)</term>
<term>Inclusion en paraffine (MeSH)</term>
<term>Microtomie (MeSH)</term>
<term>Polymères (composition chimique)</term>
<term>Populus (génétique)</term>
<term>Populus (métabolisme)</term>
<term>Protéines végétales (génétique)</term>
<term>Protéines végétales (métabolisme)</term>
<term>Solutions (MeSH)</term>
<term>Sondes d'ARN (composition chimique)</term>
<term>Substances tampon (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Fixatives</term>
<term>Formaldehyde</term>
<term>Polymers</term>
<term>RNA Probes</term>
<term>RNA, Fungal</term>
<term>RNA, Transfer</term>
<term>tert-Butyl Alcohol</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term>
<term>RNA, Messenger</term>
<term>RNA, Plant</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Plant Proteins</term>
<term>RNA, Messenger</term>
<term>RNA, Plant</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Buffers</term>
<term>Solutions</term>
</keywords>
<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>ARN de transfert</term>
<term>ARN fongique</term>
<term>Alcool tert-butylique</term>
<term>Fixateurs</term>
<term>Formaldéhyde</term>
<term>Polymères</term>
<term>Sondes d'ARN</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Eucalyptus</term>
<term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN des plantes</term>
<term>ARN messager</term>
<term>Eucalyptus</term>
<term>Populus</term>
<term>Protéines végétales</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Eucalyptus</term>
<term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>In Situ Hybridization</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN des plantes</term>
<term>ARN messager</term>
<term>Eucalyptus</term>
<term>Populus</term>
<term>Protéines végétales</term>
</keywords>
<keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Hybridation in situ</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Microtomy</term>
<term>Paraffin Embedding</term>
<term>Staining and Labeling</term>
<term>Tissue Fixation</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Coloration et marquage</term>
<term>Fixation tissulaire</term>
<term>Inclusion en paraffine</term>
<term>Microtomie</term>
<term>Solutions</term>
<term>Substances tampon</term>
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<front><div type="abstract" xml:lang="en">A non-radioactive in situ hybridization protocol is presented for localization of mRNA transcripts in thin tissue sections. In situ hybridization provides spatial resolution of transcript distribution at the cellular level that is not attained by Northern hybridization or PCR-based methods using organ- or tissue-derived RNA. With appropriately designed gene-specific probes, in situ hybridization can discriminate closely related gene family members. Although the procedure for tissue section preparation is tedious and lengthy, once prepared, the probe labeling, hybridization and signal detection steps can be easily scaled to handle multiple genes for comparative analysis.</div>
</front>
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<DateRevised><Year>2015</Year>
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<Title>Methods in cell biology</Title>
<ISOAbbreviation>Methods Cell Biol</ISOAbbreviation>
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<ArticleTitle>In situ hybridization.</ArticleTitle>
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<Abstract><AbstractText>A non-radioactive in situ hybridization protocol is presented for localization of mRNA transcripts in thin tissue sections. In situ hybridization provides spatial resolution of transcript distribution at the cellular level that is not attained by Northern hybridization or PCR-based methods using organ- or tissue-derived RNA. With appropriately designed gene-specific probes, in situ hybridization can discriminate closely related gene family members. Although the procedure for tissue section preparation is tedious and lengthy, once prepared, the probe labeling, hybridization and signal detection steps can be easily scaled to handle multiple genes for comparative analysis.</AbstractText>
<CopyrightInformation>Copyright © 2013 Elsevier Inc. All rights reserved.</CopyrightInformation>
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<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
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In situ hybridization.

In situ hybridization.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki